Surface-enhanced Raman scattering (SERS) detection of bacteria in microfluidic Dielectrophoresis (DEP)
Sepsis is a major public health concern and should be defined as life-threatening organ dysfunction caused by a dysregulated host response to infection. Even in developed country, there still have 0.2-3/1000 case in one year. The degree of infection can be defined as sepsis, severe sepsis, and septic shock and the case-fatality rate reach up to 30%, 50% and 80%, respectively. In septic shock case, each hour of delay in antimicrobial administration over the ensuing 6 hrs was associated with an average decrease in survival of 7.6% and continued drop to 25.4% by 9-12hrs. But typically detection and identification of blood samples need 29-130 hrs to obtain information on bacteria. To overcome the aforementioned problems, we propose to integrate DEP separation/concentration and SERS fingerprint identification in microfluidic chip to detect bacteria in whole blood sample within one hour. Through the bilateral cooperation to develop a set of medical significance and economic value of the fast detection/identification chip used in sepsis
Jointly supported by Mongolian Foundation for Science and Technology and Taiwan Ministry of Science and Technology. Duration of the project: July 15, 2017 – July 14, 2020.
- Principal Investigators: